Cognition biomarkers in brain (GABA, Glutamate, Beta Endorphin) and CSF (Corticosterone, KLOTHO, and Serotonin) were evaluated using unpredictable chronic stress (UCS) rodent model in presence of Consciousness Energy Healing Treated (known as the Trivedi Effect®) novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. The test formulation was consisted of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), β-carotene, ginseng, and cannabidiol isolate. The test formulation constituents were divided into two parts, one part of each ingredient was distinct as the untreated test formulation, while the other portion of the test formulation and a group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The level of brain GABA in groups viz. G7 (15-days pre-treatment of the Biofield Energy Treated Test formulation) and G8 (15-days pre-treatment of the Biofield Energy Treated Test formulation to the Biofield Energy Treatment per se rats) was increased by 12.6% and 14.3%, respectively as compared with the disease control group (G2). Brain glutamate was increased by 20.6%, 36.5%, 25.3%, and 40.7% in the G6 (Biofield Energy Treatment per se to the rats), G7, G8, and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups respectively, as compared with the G2. Brain beta endorphin was significantly increased by 114.3% and 112% in the G5 (Biofield Energy Treated Test formulation to the untreated rats) and G6 groups respectively, as compared with the G4. Similarly, the level of corticosterone in CSF was significantly decreased by 25%, 28.7%, 25%, 27.7%, and 26.9% in the G5, G6, G7, G8, and G9 groups respectively, as compared with the G2. The level of KLOTHO in CSF was significantly increased by 17.6%, 83.3%, 36.4%, and 102.8% in the G5, G6, G7, and G8 groups respectively, as compared with the G4. These data suggested that the Biofield Energy per se significantly reduce the stress-related biomarker such as corticosterone and significantly improve cognition related biomarkers such as GABA, Glutamate, Beta Endorphin, and KLOTHO protein. The brain neurotransmitters (GABA, and glutamate) level was significantly increased in the different treatment groups as compared to the disease control group, which played a vital role in animal’s cognitive functions. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in rats in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group.
