The oxidative stress plays an important role in the pathogenesis of anxiety and depression. Antioxidant assay was performed in brain tissue to evaluate the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats along with pro-inflammatory cytokines estimation using ELISA assay. The test formulation included the combination of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. All the constituents of the test formulation were divided into two parts; one portion was defined as the untreated test formulation, while the other portion of the test formulation and the animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Antioxidant assay data showed that superoxide dismutase (SOD) level was increased by 50.9%, 18.4%, 47.6%, 24%, and 27.4% in the G5 group, which was received Biofield Energy Treated Test formulation, Biofield Energy Treatment per se to the rats (G6), 15-days pre-treatment of the Biofield Energy Treated test formulation (G7), 15-days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treatment per se to rats (G8), and untreated test formulation to the Biofield Energy Treated rats (G9) groups, respectively as compared with the disease control group (G2). The level of lipid peroxidation (LPO) in the brain was significantly decreased by 20%, 21.5%, and 10% in the G5, G6, and G9 groups, respectively as compared with the disease control group (G2). However, IL-6 level in the brain was significantly decreased by 35.9%, 18.2%, 10.5%, 26.9%, and 32.6% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. The TNF-α level in brain was decreased by 73.3%, 57.2%, 63%, 80.5%, and 72.7% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. The IL-1ß level in brain was decreased by 40.2%, 37.8%, 26.7%, 43.5%, and 49.9% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G2. Overall, the data suggested that there was significant antioxidant and anti-inflammatory effect observed for the Biofield Energy per se along with preventive maintenance groups in the preclinical rat model study. Therefore, the results showed the significant slowdown the stress related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease group (G2). This energy treatment (the Trivedi Effect®) might also be beneficial against various oxidative stress-related disorders (Parkinson’s disease, Alzheimer’s disease, diabetes, and cardiovascular conditions such as high blood pressure, atherosclerosis, and stroke, etc.) and inflammatory disorders (asthma, autoimmune diseases, coeliac disease, glomerulonephritis, hepatitis, inflammatory bowel disease, etc.).
