The present study was designed to evaluate the impact of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats using unpredictable chronic stress (UCS) model for the estimation of stress hormones using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The plasma corticosterone level data showed that the level was significantly reduced by 49.8%, 75.2% (p≤0.05), 41.8%, 47.9% and 28.7% in the Biofield Energy Treated Test formulation to the untreated rats (G5), Biofield Energy Treatment per se to the rats (G6), 15 days pre-treatment of Biofield Energy Treated Test formulation (G7), 15 days pre-treatment of Biofield Energy Treated Test formulation to the Biofield Energy Treated rats (G8), and Untreated Test formulation to the Biofield Energy Treated per se rats (G9) groups, respectively as compared with the untreated test formulation group (G4). The level of plasma angiotensin-II was significantly decreased by 40.6% (p≤0.001), 46% (p≤0.001), 35.9% (p≤0.001), 21.5%, and 35.5% (p≤0.001) in the G5, G6, G7, G8, and G9 groups, respectively as compared with the G4. The level of plasma noradrenaline after treatment was reduced by 13.6%, 20.2%, and 28.4% in the G6, G7, and G8 groups, respectively compared to the G4. In addition, plasma epinephrine level after treatment was significantly reduced by 51.9% (p≤0.05), 51.9% (p≤0.05), 37.7%, 25.6%, and 35.4% in the G5, G6, G7, G8, and G9 groups, respectively compared to the G2 group. Similarly, the level of norepinephrine was measured in CSF, which was significantly decreased by 13.27%, 32.2% (p≤0.05), 19.7%, 13.7%, and 11.5% in the G5, G6, G7, G8, and G9 groups, respectively compared to the G2 group. Overall, the data suggested significance effect of Biofield Energy per se along with preventive measure on the animal with respect to various stress-related disorders. The results showed a significant slowdown of disease progression and all other disease-related complications/symptoms in the preventive Biofield Energy Treatment group per se and the Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control and untreated test formulation groups.
