This present experimental behavioral study was evaluated the study the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for the estimation of behavioral parameters using standard assay. A novel proprietary test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The each ingredient of the novel test formulation was divided into two parts; one part was defined as the untreated test formulation, while the other part of the test formulation and the animals were received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The Y-maze data suggested significant improved behavioral activity by 35% to 70% with respect to the time in start arm, time in explored arm, time in novel arm, entries in start arm, entries in novel arm, entries in explored arm, and entries in centre after treatment with the Biofield Energy Treated test formulation and Biofield Energy Treatment per se groups. The weight loaded force swim test (WL-FST) data reported with a significant increased the “swimming spent time” by 23.63%, 120.46% (p≤0.001), 58.19% (p≤0.001), 35.79%, and 29.79% in the G5, G6, G7, G8, and G9 groups, respectively as compared with the disease control (G2) group. Rotarod study showed an increased behavior (time spent) in the G6, G7, G8, and G9 groups by 16.2%, 11.8%, 25.1%, and 33.8%, respectively as compared to the G2 group. Locomotor activity was improved in the Biofield Energy Treated test formulation group (G5), G6, G7 (Biofield Energy Treated test formulation from day -15), and G8 groups with increased total number of counts using infrared actimeter by 40.82%, 42.80% (p≤0.01), 33.36%, 30.81%, and 19.73%, respectively, as compared with the G2. Knee joint pain measurement study showed improved activity in the G5, G6, and G7 groups, which showed significant increased the joint hypersensitivity in terms of gram force by 10.01%, 13.33%, and 9.17%, respectively as compared with the G4. Altogether, results envisaged that there was a significant improvement of behaviour parameters in the treatment groups. Therefore, the results showed the significant slowdown the disease progression and disease-related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.
