Unpredictable chronic stress (UCS) model was used to evaluate the effect of Consciousness Energy Healing/Blessing Treatment (the Trivedi Effect®) on a novel proprietary test formulation in male Sprague Dawley (SD) rats for the estimation of energy biomarkers by estimation of adenosine triphosphate (ATP) in brain, muscle, and liver along with stomach serotonin and citrate synthase activity using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation did not receive any treatment, while the other portion of the test formulation and three group of animals were received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of ATP in brain tissue was altered as compared with the both disease control (G2) and untreated test formulation (G4) groups. Moreover, the level of ATP in liver was increased minimally in the G7 (15-days pre-treatment of Biofield Energy Treated Test formulation) and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups, respectively as compared with the G4 group. Additionally, the ATP levels in the muscles was altered in all the treatment groups as compared to the G2 group. The level of serotonin in stomach was significantly increased by 30.8%, 44.8%, 91.2% (p≤0.05), and 36.3% in the G5, G6, G7, and G9 groups, respectively as compared with the G4. Besides, the expression of mitochondrial enzyme - citrate synthase in all the treatment groups was altered as compared to the both G2 and G4 groups. Overall, the energy biomarkers were significantly improved after Biofield Energy Treatment and could be beneficial for low metabolic energy disorders like Niemann-Pick disease, Tay-Sachs disease, Gaucher disease, Galactosemia, etc. The results also showed the significant slowdown the stress-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control group.
