The 6-Mercaptopurine, an antineoplastic agent, is useful for the treatment of many diseases. This study was performed to investigate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of 6-mercaptopurine using liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) spectroscopy. The 6-mercaptopurine sample was divided into two parts - one part was considered as a control sample (i.e., no Biofield Energy Treatment was provided), while the other part was considered as a treated sample (i.e., treated with The Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton). The LC-MS spectra of both the samples at retention time (Rt) 2.24 minutes exhibited the mass of the protonated molecular ion peak at m/z 153 [M+H] + (calculated for C5H5N4S+, 153.18). The LC-MS based isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 33S/32S) in the treated 6-mercaptopurine was significantly decreased by 42.49% compared with the control sample. Thus, 13C, 2H, 15N, and 33S contributions from (C5H5N4S) + to m/z 154 in the treated sample were significantly decreased compared with the control sample. The GC-MS based isotopic abundance ratio of PM+1/PM in the treated 6-mercaptopurine was decreased by 1.34% compared with the control sample. Hence, 13C, 2H, 15N, and 33S contributions from (C5H5N4S)+ to m/z 153 in the treated sample were significantly decreased compared with the control sample. However, the isotopic abundance ratio of PM+2/PM in the treated 6-mercaptopurine was significantly increased by 11.58% compared with the control sample. Therefore, 18O contributions from (C5H5N4S) + to m/z 154 in the treated sample were significantly increased compared with the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 33S/32S) and PM+2/PM (34S/32S) in the treated 6-mercaptopurine was significantly altered compared to the control sample. It can be assumed that the changes in isotopic abundance and mass peak intensities could be due to changes in nuclei possibly through the interference of neutrino particles via The Trivedi Effect® - Consciousness Energy Healing Treatment. The new form of treated 6-mercaptopurine would be better in designing novel pharmaceutical formulations that might offer a better therapeutic response against acute lymphocytic leukemia, chronic myeloid leukemia, Crohn's disease, ulcerative colitis, etc.
