Silver sulfadiazine is a topical antibiotic helps to prevent and treat wound infections in patients with severe burns. This research was performed to evaluate the impact of the Trivedi Effect® - Consciousness Energy Healing Treatment on the structural properties and the isotopic abundance ratio of silver sulfadiazine using spectroscopic and chromatographic techniques. The test sample silver sulfadiazine sample was divided into control and treated parts. Only the treated part of silver sulfadiazine was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the samples at retention time (Rt) 4.1 minutes exhibited the mass of the protonated molecular ion peak at m/z 251.08 (calculated for C10H11N4O2S+, 251.06) was found to be sulfadiazine. The chromatographic peak area of the treated silver sulfadiazine was significantly increased by 20.35% compared to the control sample. The result indicated that the solubility profile of the treated silver sulfadiazine must have increased significantly after the Biofield Energy Treatment compared with the control sample. The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfadiazine was significantly increased by 31.88% compared with the control sample. Similarly, the GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfadiazine was significantly increased by 9.74% and 15.13%, respectively compared with the control sample. Hence, 13C, 2H, 15N, 17O, 33S, and 34S contributions from (C7H9N2O2S)+ to m/z 186 and 187 in the treated sulfadiazine were significantly increased compared to the control sample. Thus, the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (34S/32S) in the treated sulfadiazine were significantly increased compared to the control sample. The changes in isotopic abundance and peak area might be due to changes in nuclei possibly via the interference of neutrino particles controlled by the Trivedi Effect®-Consciousness Energy Healing Treatment. The increased isotopic abundance ratio of the treated silver sulfadiazine might improve the chemical bond strength, led to increase the stability in the body. This novel silver sulfadiazine would be more efficacious in pharmaceutical formulations which might offer better solubility, bioavailability, and therapeutic response against bacterial infections, superficial and partial thickness burn injuries, etc.
