Silver sulfadiazine is a sulfonamide-based topical antibiotic used in the partial and full burn to prevent infection. This study was performed to investigate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of silver sulfadiazine using LC-MS and GC-MS spectroscopy. Silver sulfadiazine sample was divided into two parts, one part of silver sulfadiazine was considered as control (no Biofield Energy Treatment was provided), while the second part was treated with The Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton and termed as a treated sample. The LC-MS spectra of both the control and Biofield Energy Treated samples at retention time (Rt) 4.1 minutes exhibited the mass of the protonated molecular ion peak at m/z 251.08 (calculated for C10H11N4O2S+, 251.06) with 100% base peak intensity in the MS spectrum in +ve ion mode was found to be sulfadiazine. The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfadiazine was significantly increased by 59.03% compared with the control sample. Thus, 13C, 2H, 15N, 17O, and 33S contributions from (C10H11N4O2S)+ to m/z 252.08 in the treated sample were significantly increased compared with the control sample. The control and treated silver sulfadiazine showed the presence of the chromatographic peak at the retention time of 17.71 min in the GC-MS chromatograms. The GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfadiazine was significantly decreased by 3% and 22.04%, respectively compared with the control sample. Hence, 13C, 2H, 15N, 17O, 33 S, and 34S contributions from (C7H9N2O2S)+ to m/z 186 and 187 in the Biofield Energy Treated sample were significantly decreased compared with the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (34S/32S) in the treated silver sulfadiazine were significantly decreased compared to the control sample. It can be assumed that the changes in isotopic abundance and mass peak intensities could be due to changes in nuclei possibly via the interference of neutrino particles controlled by The Trivedi Effect® - Consciousness Energy Healing Treatment. The new form of silver sulfadiazine would be better for designing novel pharmaceutical formulations that might offer better therapeutic response against bacterial infections, superficial and partial thickness burn injuries, etc.
