The aim of the present study was to evaluate the fundamental physiological functional biomarkers analysis of biofield treated/blessed novel test formulation in Unpredictable Chronic Stress (UCS) male Sprague Dawley (SD) rats. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other part of each components and three group of animals received biofield energy healing/blessing treatment by Mr. Mahendra Kumar Trivedi, a famous biofield energy healer. Total Leucocyte Count (TLC) count was significantly (p≤0.001) improved by 75.62%, 64.20%, 44.03%, 82.53%, and 50.83% in the biofield energy treated test formulation to the untreated rats (G5), biofield energy treatment per se to the rats (G6) groups, 15 days pre-treatment of biofield energy treated test formulation (G7), 15 days pre-treatment of biofield energy treated test formulation to the biofield energy treatment per se to rats (G8), and untreated test formulation to the biofield energy treated rats (G9) respectively, as compared with the G2 group. Besides, lymphocytes count were significantly increased by 52.50% (p≤0.001), 47.02% (p≤0.001), 32.21%, 83.86% (p≤0.001), and 40.15% (p≤0.001) in the G5, G6, G7, G8, and G9 groups, correspondingly with reference to G2 group. In addition, monocyte counts were also significantly increased by 82.52% (p≤0.05), 65.06% (p≤0.05), 38.68%, and 17.03% in the G5, G6, G8, and G9 groups, respectively as compared with the G2 group. Biochemical analysis showed that glucose level was maintained in all the experimental test groups, while UCS significantly increased the level of glucose. Biochemical analysis showed that HDL level was significantly (p≤0.001) improved by 37.62%, 46.64%, and 36.27% in the G5, G7, and G9 groups, respectively as compared with the G2. However, total cholesterol was decreased by 14.86%, 30.32%, 44.29%, and 32.66% in the G5, G7, G8, and G9 groups, correspondingly with reference to G2. However, VLDL level was also significantly reduced by 14.91%, 30.70%, 44.30% (p≤0.01), and 32.89% in the G5, G7, G8, and G9 groups, respectively as compared with the G2. Hepatic and cardiac biomarkers analysis showed that ALP level was decreased by 27.97% and 13.37% in the G5 and G9 groups, respectively as compared with the G2. The level of SGOT was significantly (p≤0.01) decreased by 21.45%, 19.19%, and 19.81% in the G5, G7, and G9 groups, respectively as compared with the G2. On the other hand, SGPT level was also altered by 11.32% and 11.72% in the G5 and G6 groups, respectively with reference to G2 group. CK-MB level was significantly reduced by 16.32% and 37.01% (p≤0.01) in the G8 and G9 groups, respectively with reference to G2. Animal weight parameters like body weight, organ to body weight ratio, and feed intake data showed that the changes were non-significant and no toxic effect was observed after the experimental period. Overall, the data suggested a significant improvement of immune-related hematology parameters, CK-MB, SGOT, and lipid profile after biofield treatment and slowdown the disease progression rate and related symptoms in the preventive maintenance groups. Therefore, the data could be benefitted to build-up good body immune responses, enhance resistance towards diseases, and reduce allergies and lethargic conditions.
