The study was aimed to evaluate the anti-inflammatory potential of the Trivedi Effect® - Biofield Energy Healing Treatment on test formulation in male Sprague Dawley rats. Each ingredient of test formulation was divided in two portions. One part was denoted as the control without Biofield Treatment, while another part and three groups of animals were received Biofield Treatment by Mr. Mahendra Kumar Trivedi. Results showed that level of tumor necrosis factor-α (TNF-α) was significantly reduced by 11.37%, 11.19%, and 62.6% in Biofield Treated test formulation group (G5), Biofield Energy Treated test formulation at day -15 group (G7), and Biofield Treatment per se to animals plus Biofield Treated test formulation from day -15 groups (G8), respectively as compared to untreated test formulation (G4). Interleukin-1β (IL-1β) level was significantly decreased by 41.77% (p≤0.001), 28.76%, 64.44% (p≤0.001), and 75.65% (p≤0.001) in G6 (Biofield Energy Treatment per se at day -15), G7, G8, and Biofield Treatment per se (day -15) to animals plus untreated test formulation (G9) groups, respectively compared to G4. Moreover, IL-6 level was significantly reduced by 18.97%, 43.44% (p≤0.05), 17.43%, 15.37%, and 28.50% in the G5, G6, G7, G8, and G9 groups, respectively compared to G2. Besides, monocyte was significantly increased by 30.77% and 23.08% in the G6 and G7 groups, respectively; while neutrophil was increased by 21.52% in G8 as compared to G2. Further, eosinophil level was decreased by 20.67%, 33.33%, and 16.67% in G6, G8, and G9 groups, respectively compared to G4. Testosterone was significantly increased by 98.11% and 311.43% (p≤0.001) in G5 and G6 groups, respectively compared to G2. Overall, results suggested that Biofield Energy Treated test formulation and Biofield Energy Treatment per se to animals improved inflammation-related parameters and can be used for autoimmune and inflammatory disorders, stress management, and anti-aging by improving overall health.
