Vitamin D receptor (VDR) expression and telomerase enzyme activity was performed in the unpredictable chronic stress (UCS) model for the evaluation of Consciousness Energy Healing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats using ELISA assay. A unique test formulation of minerals (Zn, Fe, Cu, Se, Ca, Mg), vitamins (C, E, B6, B12, D3), and nutraceuticals (β-carotene, ginseng, and cannabidiol isolate) was formulated. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other portion of the test formulation and three group of animals were received Biofield Energy Healing Treatment by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. VDR in liver showed an increased expression in the Biofield Energy Treated Test formulation to the untreated rats (G5), G7 (15-days pre-treatment of Biofield Energy Treated Test formulation), and G8 (15 days pre-treatment of Biofield Energy Treated test formulation to the Biofield Energy Treatment per se rats) groups by 16.7%, 75.7%, and 14.3%, respectively as compared with the untreated test formulation group (G4). Similarly, the VDR expression in kidney was increased by 22.3% and 13.6% in the G6 (Biofield Energy Treatment per se to the rats) and G8 groups, respectively, as compared with the G4. However, the VDR expression in heart was altered by 20.2%, 26%, and 28.2% in the G5, G7, and G8 groups respectively, as compared with the G4. The telomerase activity in the brain was significantly increased by 16.9%, 116%, 258.6% (p≤0.01), 148.1%, and 54.7% in the G5, G6, G7, G8, and G9 (untreated test formulation to the Biofield Energy Treatment per se to the rats) groups respectively, as compared with the G4. Similarly, telomerase activity in heart was also increased by 11.1% in the G8 group as compared with the G4. Overall, the experimental data suggested significance effect of Biofield Energy per se along with preventive measure on the animal with respect to various stress-related disorders. Overall, the results showed the significant slowdown the stress-related disease progression and its complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) as compared to the disease control and untreated test formulation groups.
