Combined test formulations including extract, minerals and vitamins are extensively used worldwide for wide range of disorders. A novel proprietary formulation was designed that consist of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. This formulation was tested and evaluated for the impact of Consciousness Energy Healing/Blessing Treatment (the Trivedi Effect®) on a novel test formulation in male Sprague Dawley (SD) rats, fed with vitamin D3 deficiency diet (VDD) for immunomodulatory activity. The novel test formulation was divided into two parts, one section was defined as the untreated test formulation, while the other part was defined as the Biofield Energy Treated/Blessed sample, which received the Biofield Energy Healing Treatment/Blessing by renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. Platelet counts were increased by 12.43% in the Biofield Energy Treated test formulation from day -15 (G7) group as compared with the G4 group. Total cholesterol was decreased by 15.01%, 12.34%, and 14.80% was reported in the G5, G6, and G9 groups, respectively as compared with the G4 group. The level of LDL was significantly reduced by 40.66% (p≤0.05), 33.38% (p≤0.05), 36.75% (p≤0.05), 27.37%, and 20.54% in the test groups viz. G5, G6, G7, G8, and G9 groups, respectively as compared with the G4 group. VLDL was significantly decreased by 10.31% and 37.63% (p≤0.01) in the G5 and G6 groups, respectively as compared with the G4. The level of triglycerides was significantly (p≤0.01) reduced by 38.13% in the G6 group as compared with the G4 group. Calcium level was significantly (p≤0.001) improved by 16.01%, 18.91%, and 14.7% in the G5, G7, and G8 groups, respectively as compared with the G4 group. Hormonal profile data suggested that testosterone was showed increased by 603.56%, 155.39%, 334.30%, 174.24%, and 224.39% in the G5, G6, G7, G8, and G9 groups, respectively while, serum corticosterone level was reported to be decreased by 13.94%, 23.61%, and 22.13% in the G6, G8, and G9 groups, respectively as compared with the G4 group. Organ histopathological analysis suggested that findings were spontaneous or incidental in nature, representing the normal physiological/metabolic or congenital changes encountered all the experimental groups. Altogether, the Biofield Treated test formulation and Biofield Energy Treatment per se significantly improved the immune-related parameters along with hormones like testosterone and corticosterone, which might be beneficial for the management of immune-compromised patients as well as to boost-up the immunity in healthy peoples. Overall, the results showed the significant slowdown the disease progression and disease-related all other complications/symptoms in the preventive Biofield Energy Treatment group per se and/or Biofield Energy Treated Test formulation groups (viz. G6, G7, G8, and G9) comparatively with the disease control group.
