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Basic Phage Electron Microscopy

The page contains an abstract and two images of bacteriophages under an electron microscope. The abstract states that negative staining of purified viruses is the most important electron microscope technique in virology. The mail stans are phosphotungstate and uranyl acetate which both have problems and advantages. Specific problems are encountered in photography, calibration of magnification, measurements, and interpretations of...

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Keywords:
phosphotungstate, negative staining, microscopical, virology, bacteriophages, uranyl acetate, purified viruses, stain
Disciplines:
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More about this material

Material Type:
Learning Object Repository
Date Added to MERLOT:
July 13, 2024
Date Modified in MERLOT:
August 16, 2024
Author:
Submitter:
A. Zheng
Primary Audience:
College Lower Division, College Upper Division, Graduate School
Technical Format:
Image, Website
Mobile Compatibility:
Not specified at this time
Language:
English
Cost Involved:
No
Source Code Available:
Yes
Creative Commons:
Unknown

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Comments

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M Zhou
M Zhou (Student)
1 year ago
This page describes an important procedure in phage electron microscopy, negative staining. Electron microscopy is a technique that uses a focused beam of electrons to image a specimen. This page also describes 2 different staining options: phosphotungstate and uranyl acetate, explaining that they both have their pros and cons. There can also be problems with photography, calibration, measurements, and interpretation when using phage electron microscopy