Pyridoxine (vitamin B6) is commonly found in foods and dietary supplements, has an important role in physiologic development. In this study, the influence of the Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of vitamin B6 were evaluated using LC-MS and GC-MS. Vitamin B6 sample was divided into two parts and termed as control and treated samples. Only the treated vitamin B6 was received the Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Dahryn Trivedi. The LC-MS spectra of both the vitamin B6 samples at retention time 2.3 min exhibited the mass of the protonated molecular ion peak [M+H]+ at m/z 170 (calculated for C8H12NO3+, 170.08) in the MS spectrum. The peak area% of the treated vitamin B6 was increased by 1.32% compared to the control sample. The isotopic abundance ratio of PM+1/PM in the treated vitamin B6 was significantly decreased by 36.94% compared with the control sample. Thus, 13C, 2H, 15N, and 17O contributions from (C8H12NO3)+ to m/z 171 in the treated sample were significantly decreased compared with the control sample. Similarly, in the GC-MS chromatograms, the peak area of the treated sample was increased by 4.54% compared to the control sample. Similarly, the mass peak intensity of the treated vitamin B6 at m/z 153 was significantly increased by 66.69% compared to the control sample. The isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O) and peak area in the treated vitamin B6 was significantly decreased compared to the control sample. The decreased isotopic abundance ratio of the treated vitamin B6 may decrease the intra-atomic bond strength, decrease its physical stability. The increased peak area of the treated vitamin B6 might be responsible for the increase in solubility and bioavailability compared to the control sample. The new form of vitamin B6 would be better for designing more efficacious pharmaceutical formulations for the prevention and treatment of vitamin B6 deficiency, anaemia, seizures, cardiovascular disease, tuberculosis, Alzheimer’s disease, cancer, anxiety, hypertension, asthma, depression, dysmenorrhea, breast pain, etc.
