Sulfamethoxazole is a broad spectrum antibiotic especially used for the treatment of infections caused by bacteria. This study was performed to investigate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment on the structural properties and the isotopic abundance ratio of sulfamethoxazole using LC-MS and GC-MS spectroscopy. Sulfamethoxazole sample was divided into two parts, one part of sulfamethoxazole was considered as control (no Biofield Energy Treatment was provided), while the second part was treated with The Trivedi Effect®-Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton and termed as a treated sample. The LC-MS spectra of both the samples at retention time (Rt) 2.5 minutes exhibited the mass of the deprotonated molecular ion peak at m/z 252 [M-H]- (calculated for C10H10N3O3S-, 252.04). The LC-MS based isotopic abundance ratio of PM+1/PM in the treated sulfamethoxazole was significantly increased by 44.67% compared with the control sample. Thus, 13C, 2H, 15N, 17O, and 33S contributions from (C10H10N3O3S)- to m/z 253 in the treated sample were significantly increased compared with the control sample. The control and treated sulfamethoxazole showed the presence of a chromatographic peak at the retention time of 16.91 min in the GC-MS chromatograms. The peak area% of the treated sample was significantly increased by 80.3% compared to the control sample. The GC-MS based isotopic abundance ratio of PM+1/PM and PM+2/PM in the treated sulfamethoxazole was significantly increased by 24.13% and 90.53%, respectively compared with the control sample. Hence, 13C, 2H, 15N, 17O, 18O, 33S, and 34S contributions from (C10H10N3O3S)+ to m/z 254 and 255 in the Biofield Energy Treated sample were significantly increased compared with the control sample. The isotopic abundance ratios of PM+2/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (18O/16O or 34S/32S) in the treated sulfamethoxazole were significantly altered compared to the control sample. It can be assumed that the changes in isotopic abundance and mass peak intensities could be due to changes in nuclei possibly through the interference of neutrino particles via The Trivedi Effect®- Consciousness Energy Healing Treatment. The new form of sulfamethoxazole would be better designing novel pharmaceutical formulations that might offer better solubility, dissolution, absorption, bioavailability and therapeutic response against urinary tract infections, ear infections, tuberculosis, traveler’s diarrhea, shigellosis, bronchitis, and pneumocystis jiroveci pneumonia, etc.
