Cefazolin sodium is a broad-spectrum beta-lactam antibiotic and first-generation cephalosporin with bactericidal activity. In this study, the impact of the Biofield Energy Healing/Blessing on cefazolin sodium for the assessment of isotopic abundance ratio by LC-MS and GC-MS spectroscopy. For this study, the test sample cefazolin sodium was divided into two parts, control and treated sample. The control test sample did not receive any Blessing, but the treated test item was received the Biofield Treatment/Blessing remotely by Mr. Mahendra Kumar Trivedi, a renowned Spiritual Biofield Energy Healer, The LC-MS spectra of both the test items at the retention time (Rt) 4.7 min exposed the mass of the protonated molecular ion peak at m/z 455 [M+H]+. The Biofield Treated cefazolin sodium was significantly increased (67.66%) the isotopic abundance ratio of PM+1/PM (13C/12C or 2H/1H or 17O/16O or 15N/14N or 33S/32S) by LC-MS than untreated cefazolin. Therefore, 13C, 2H, 15N, 17O, and 33S contributions from (C14H15N8O4S3)+ to m/z 456 were increased significantly in the Biofield Treated/Blessed cefazolin than untreated sample. The GC-MS peak intensities of the treated test item at m/z 56 and 132 were significantly raised by 130.15% and 188.4%, respectively compared to the control sample. The increased isotopic abundance ratio in the Biofield treated/Blessed cefazolin sodium may increase the intra-atomic bond strength and its physical stability. The new form of cefazolin sodium would be better to design more efficacious pharmaceutical formulations against urinary tract infections, respiratory tract infections, cellulitis, endocarditis, pneumonia, joint infection, biliary tract infections, blood infections, genital infections, and also prevent group B streptococcal disease at the time of delivery and before surgery, etc.
