Thiamine (vitamin B1) is an essential water soluble vitamin required in all living cells for many cellular functions. The objective of the study was to investigate the impact of the Trivedi Effect®-Consciousness Energy Healing Treatment on the isotopic abundance ratios (PM+1/PM and PM+2/PM) and structural properties of thiamine hydrochloride using LC-MS, GC-MS, and NMR spectroscopy. Thiamine sample was divided into two parts, one part of the sample was termed as untreated/control thiamine, while the other part of the sample received the Trivedi Effect®-Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi and designated as Biofield Energy Treated thiamine. The LC-ESI-MS analysis of both the thiamine samples showed the mass of parent molecular ion at m/z 265.03 (calcd for C12H17N4OS+, 265.11) at the retention times (Rt) 1.51 minutes with similar fragmentation pattern. But, the relative peak intensities of the treated sample were significantly improved compared to the control sample. The isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O or 33S/32S) and PM+2/PM (18O/16O or 34S/32S) were significantly increased by 43.60% and 42.68%, respectively in the treated thiamine compared to the control sample. Therefore, 2H, 13C, 15N, 17O, and 33S contributions from C12H17N4OS+ to the isotopic m/z 266 and 18O and 34S contributions to the isotopic m/z 267 was significantly increased in the treated sample compared with the control sample. The GC-MS mass peak intensity of the treated sample (52940.7) was significantly increased by 131.01% compared to the control sample (22917.04). The proton and carbon signals for CH3, CH2, NH2, CH, =C=, and C-OH groups in the 1H and 13C NMR spectra of the control and treated samples were similar. The increased mass peak intensities and isotopic abundance ratios in thiamine might be due to the Trivedi Effect®-Consciousness Energy Healing Treatment via the possible mediation of neutrinos, which may change the intra-atomic bond strength, its physical stability, kinetic isotope effects of the treated thiamine. Thus, the Trivedi Effect®-Consciousness Energy Healing Treated thiamine would be advantageous for designing better nutraceuticals and pharmaceutical formulations which might provide better therapeutic response against vitamin B1 deficiency and related disorders, including beriberi, Korsakoff’s syndrome, Parkinson’s and Alzheimer’s diseases, maple syrup urine disease, Leigh’s disease, optic neuropathy, alcoholism, and aging.
