The aim of this study was to evaluate the effect of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on immune biomarkers and testosterone on Nω-Nitro-L-arginine Methyl Ester (L-NAME) and high fat diet (HFD)-induced cardiovascular disorders in male Sprague Dawley rats. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, cyanocobalamin, and cholecalciferol), Panax ginseng extract, β-carotene, and cannabidiol isolate. In this experiment, nine groups were assigned, in which four were preventive maintenance groups. The constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation, while the other part and three group of animals received Biofield Energy Healing Treatment/Blessing remotely for about 3 minutes by a renowned Spiritual Energy Healer, Mr. Mahendra Kumar Trivedi. The results showed that cluster of differentiation 4 (CD4+)-T-cell count was significantly increased by 11.45%, 20.02%, 14.18% (p≤0.05), 19.37% (p≤0.05), and 10.53% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15), G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15), and G9 (L-NAME + HFD along with Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively as compared to the untreated test formulation (G4) group. Moreover, the CD8+ T-cell count was increased by 13.82% in the G8 group as compared to the G2 group. There was an alteration of immunoglobulins in the treatment groups than G2. The level of testosterone was significantly increased by 55.53%, 161.66% (p≤0.05), 110.03% (p≤0.05), 93.71% (p≤0.05), and 102.05% (p≤0.05) in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Hematological parameter like platelet count was increased by 10.18%, 10.44%, 14.23%, and 14.44% in the G5, G7, G8, and G9 groups, respectively as compared to the G2 group. Additionally, RBC distribution width-variation coefficient (RDW-CV) was altered by 13.29%, 16.94%, 14.83%, and 11.55% in the G5, G6, G8, and G9 group respectively, as compared with the G2 group. Histopathological examination data did not show any drastic cellular changes in all the treatment groups. Overall, the data suggested that “Trivedi’s Biofield” significantly improved the cellular immune biomarkers, testosterone, and hematology parameters in the preventive treatment groups with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the study outcomes could be slowdown the cardiovascular disease progression rate and its complications in the preventive treatment groups (viz. G6, G7, G8, and G9).
