The study was planned to evaluate the effect of the Trivedi Effect®- Biofield Energy Treated Test formulation (TI) composed of minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, alpha tocopherol, cyanocobalamin, and cholecalciferol), Panax ginseng extract, CBD isolates, and β-carotene on senescence of human lung fibroblasts (WI-38) cell line in EMEM medium. Growth rate (amount of doubling in one unit of time) was determined, while senescence was induced by H2O2 damage and then cells were allowed to recover for 48 h. The test formulation constituents of the test formulation were divided into two parts; one section was defined as the untreated test formulation (UT), while the other portion of test formulation received Biofield Energy Healing/Blessing Treatment (BT) by a renowned Biofield Energy Healer, Mr. Mahendra Kumar Trivedi. The test items were treated with Biofield Energy Healing Treatment and divided as Biofield Energy Treated (BT) and untreated (UT) test items. MTT data showed that the test formulation in various tested concentrations was found as safe and nontoxic with viability range 70% to 125%. The decreased reduction in doubling time was calculated on the basis of untreated test group of EMEM and test formulation. The UT-EMEM + BT-TI test group reported with decreased doubling time by 67% and 67.5% at 0.001 and 0.1 µg/mL, respectively, while 134.3%, 24.4%, and 50.5% decreased doubling time was found in the BT-EMEM + UT-TI group at 0.001, 0.01, and 0.1 µg/mL respectively, as compared with untreated test group. In addition, BT-EMEM + BT-TI test group showed a significant reduction in doubling time value by 191.6%, 33.2%, and 74.6% at 0.001, 0.01, and 0.1 µg/mL respectively, as compared with the UT-EMEM + UT-TI group. Overall, the results showed the significant decreased doubling time in WI-38 cells. Thus, the mechanical properties of cells such as cellular function, including shape, motility, differentiation, division, and adhesion to its surrounding extracellular matrix were improved. Overall, it can be useful in many disease progressions with significant anti-aging activity and its associated complications/symptoms.
