The experiment was aimed to evaluate the effect of Biofield Energy Treated/Blessed Proprietary Test Formulation and Biofield Energy Treatment/Blessing per se on different vital functional serum biomarkers to the animals on L-NAME and high fat diet (HFD)-induced cardiovascular disorders in Sprague Dawley rats. In this experiment, the functional serum biomarkers such as epinephrine/adrenaline, inducible nitric oxide synthase (iNOS), endothelial NOS (eNOS), plasminogen activator inhibitor-1 (PAI-1), intercellular adhesion molecule-1 (ICAM-1), glycated hemoglobin (HbA1c), and insulin were measured using ELISA assay. A test formulation was formulated including minerals (magnesium, zinc, copper, calcium, selenium, and iron), vitamins (ascorbic acid, pyridoxine HCl, vitamin B9, vitamin B12, and vitamin D3), cannabidiol (CBD) isolate, Panax ginseng extract, and β-carotene. The constituents of the test formulation were divided into two parts; one portion was referred as the untreated test formulation, while the other portion and three groups of animals received Biofield Energy Healing/Blessing Treatment remotely for about 3 minutes by a renowned spiritual healer, Mr. Mahendra Kumar Trivedi. The results showed that the level of adrenaline was reduced by 29.25%, 20.33%, 31.36%, and 41.97% in the G5 (L-NAME + HFD + the Biofield Energy Treated test formulation), G6 (L-NAME + HFD + Biofield Energy Treatment per se to animals from day -15), G7 (L-NAME + HFD + the Biofield Energy Treated test formulation from day -15), and G8 (L-NAME + HFD + Biofield Energy Treatment per se plus the Biofield Energy Treated test formulation from day -15) groups, respectively as compared to the untreated test formulation group (G4). Moreover, the level of iNOS was reduced by 53.09%, 51.32%, 33.87%, 39.88%, and 40.02% in the G5, G6, G7, G8, and G9 (L-NAME + HFD + Biofield Energy Treatment per se animals plus the untreated test formulation) groups, respectively, as compared to the disease control group (G2). Additionally, the level of eNOS was increased by 83.65%, 94.93%, 71.48%, 48.64%, and 61.12% in the G5, G6, G7, G8, and G9 groups, respectively, as compared to the G2 group. The level of PAI-1 was decreased by 56.10%, 56.57%, 55.28%, 49.28%, and 57.84% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Besides, the level of ICAM-1 was decreased by 20.99%, 20.74%, 18.85%, and 17.89% in the G5, G6, G7, and G8 groups, respectively as compared to the G2 group. Level of HbA1c was decreased by 28.50%, 44.07%, 25.30%, 22.70%, and 48.79% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Further, the level of insulin was decreased by 26.09%, 21.24%, 5.41%, 23.61%, and 24.59% in the G5, G6, G7, G8, and G9 groups, respectively as compared to the G2 group. Overall, the data suggested significance improvement of vital functional biomarkers of the Biofield Energy Treated test formulation and Biofield Energy Treatment per se along with preventive measure on the animal with respect to various pathological conditions that might be beneficial various types of cardiovascular disorders. Therefore, the results showed the significant slowdown the inflammation-related cardiovascular disease progression and its symptoms in the preventive maintenance groups (viz. G6, G7, G8, and G9).
