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Activity 6:  Recombinant DNA Techniques

Activity 6: Recombinant DNA Techniques

This site simulates a a paper cut out insertion of the human insulin gene into a plasmid, requiring a choice of which restriction endonuclease to use.


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Thomas Lonergan
Thomas Lonergan (Faculty)
20 years ago
While this is not a flashy site, the activity suggested is excellent. Users of
this activity make a paper cutout of a circular plasmid, and insert a paper
cutout of the human insulin gene. User must choose the appropriate restricion
enzyme from a provided list. I have used this activity in a sophomore cell and
molecular biology class as well as for high school teacher training classes.
The questions that are raised by this activity are excellent. It is a good
activity to consider prior to using restriction enzymes in a laboratory setting.