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The objective of the present study was to evaluate the immunomodulatory effect of Biofield Energy Treatment (The Trivedi Effect®) on Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) in murine macrophage cells (RAW 264.7). The study parameters were evaluated using cell viability by MTT assay and estimation of proinflammatory cytokine like tumor necrosis factor - alpha (TNF-α) on immunomodulation using enzyme linked immune sorbent assay (ELISA). The cell viability using MTT assay data showed that more than 80% cell viability was observed in all the tested groups compared to the baseline control group (G1). The characteristics of cell morphology in the Biofield Energy Treated DMEM without FBS (G6) group showed some changes in the cell morphology as evidenced a transition from spherical to elongate and also showed maturation compared to the G1 group. The level of TNF-α expression was significantly reduced by 23.15% in the G3 group compared to the G1 group. Moreover, the secretion of TNF-α was altered by 38.06%, 35.77%, and 282.49% in the G4, G5, and G6 groups, respectively compared to the G1 group. The overall results demonstrated that the Trivedi Effect® - Consciousness Energy Healing Treatment has an impact on DMEM and FBS by alteration of cell morphology and reducing the level of TNF-α expression in immune cell (RAW 264.7). Therefore, the Trivedi Effect® treated DMEM and FBS might be useful as an immunomodulator for various immune-related disorders like Graves’ disease, rheumatoid arthritis, multiple sclerosis, etc.

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