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Hair plays a lucrative outlook on the human body and also contribute an exciting part in social and sexual communication. Loss of hair follicle would potentially lead to various skin disorders. For this consequence, the present study was investigated for the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by a renowned Biofield Energy Healer Alice Branton. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20% and 26.67% increased bulb thickness on day 5 and 7, respectively compared to day 1. Besides, the percent telogen follicle was found as 86%, 86%, and 100% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via an increased formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc.

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