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Lactase Enzyme Lab

A simple classroom lab which uses the enzyme Lactase to demonstrate the specificity of enzymes to a substrate (in this case, Lactose).  Also, demonstrates how heat can denature enzymes.  Glucose test strips are used to test for the presence of this sugar in milk, and a simple sugar solution, before and after lactase has been added.

Keywords:
biology, chemical reaction, denature, lactose, lactase, lab, enzyme
Disciplines:
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More about this material

Material Type:
Assignment
Date Added to MERLOT:
September 4, 2009
Date Modified in MERLOT:
December 10, 2019
Author:
Submitter:
Elizabeth Stephens
Asian American, Native American, Pacific Islander Serving Institution Hispanic Serving Institution
Primary Audience:
High School, College General Ed, College Lower Division
Technical Format:
PDF, Website
Mobile Compatibility:
Not specified at this time
Language:
English
Cost Involved:
No
Source Code Available:
Unknown
Accessibility Information Available:
Unknown
Creative Commons:
No

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Comments

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Anil Kapoor
Anil Kapoor (Faculty)
9 years ago

Great lab, I am planning on using this in the classroom in the spring

Time spent reviewing site: 35 min
Shirley Baker
Shirley Baker (Student)
12 years ago
I would definitely be interested in using this lab as an adjunct to teaching enzymes in the macromolecules unit in Biology. It also reviews monosaccharides and disaccharides as well. Good example of scientific method, how a ‘control’ is made, and how enzymes are specific for their substrates.