L-tryptophan is an essential amino acid that helps in growth in infants and aids nitrogen balancing in adults, etc. This study helps in evaluating the impact of the Trivedi Effect® on the physicochemical and thermal properties of L-tryptophan with the help of various analytical techniques. One part of L-tryptophan sample didn’t receive any treatment and was called the control sample, while the other part was given the Biofield Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and named as the treated sample. The particle size values were significantly altered by -43.95%(d10), -6.28%(d50), 9.97%(d90), and 10.36%[D(4,3)]; thus, the specific surface area was significantly increased by 51.61% in the treated sample compared to the control sample. The PXRD peak intensities and crystallite sizes were significantly altered ranging from -61.17% to 385.06% and -48.52% to 3.76% respectively, however, the average crystallite size was significantly reduced by 26.03% in the treated sample compared to the control sample. The total weight loss was significantly increased by 17.35%; additionally, the residual amount was significantly reduced by 83.95% in the treated sample compared to the control sample. The ΔHfusion was significantly increased by 10.43% in the treated sample compared to the control sample. Thus, the Biofield Treatment produced the novel polymorph of L-tryptophan that might give better solubility, bioavailability, and thermal stability in nutraceutical/pharmaceutical formulations with and an improved drug profile.