A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Energy of Consciousness Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without Biofield Energy Healing Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals was also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The IgM, IgG, CD4+, and CD8+ were altered in the Biofield Energy Treated test formulation (G4) compared to the disease control (G2). TLC was significantly increased by 19.35% in the G4 compared to the G2. Neutrophil was significantly increased by 31.53%, 29.73%, 31.53%, and 33.84% in the G4, untreated test formulation (G5), G6, and G7, respectively compared to the G2. The levels of total cholesterol (TC), triglycerides (TG), and very low density lipoprotein (VLDL) were significantly lowered by 18.18% (p≤0.01), 46.33% (p≤0.01), and 46.41% (p≤0.01), respectively in the G4 compared to the G2. Additionally, the levels of TC, TG, low density lipoprotein (LDL), and VLDL were significantly reduced by 25.50% (p≤0.001), 96.39% (p≤0.001), 13.91%, and 55.08% (p≤0.001), respectively in the G6 compared to the G2. Alkaline phosphatase (ALP) was significantly reduced by 33.65% (p≤0.001) in the G4 compared to the G2. Moreover, the levels of serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), ALP, and creatine kinase myocardium band (CK-MB) were significantly decreased by 29.44% (p≤0.05), 25.54% (p≤0.01), 27.97% (p≤0.01), and 36.07% (p≤0.01), respectively in the G7 group compared to the G2. Testosterone was significantly increased by 83.68%, 305.90%, 1271.03% (p<0.001) and 745.35% (p<0.05) in the G4, G5, G6 and G7, respectively compared to the G2. The level of catalase enzyme was significantly increased by 57.91% (p<0.01) and 98.51% (p<0.001) in Biofield Energy Treatment per se at day -15 (G6) and Biofield Energy Treated test formulation at day -15 (G7), respectively compared to the G2. Overall, results suggested that the Biofield Energy Treated herbomineral formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.
