Pyridoxine hydrochloride is water-soluble B vitamin widely used for the prevention and treatment of vitamin B6 deficiency and other several diseases. The aim of this study was to explore the effect of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the physicochemical and thermal properties of pyridoxine hydrochloride using PXRD, PSA, DSC, and TGA/DTG analysis. Pyridoxine hydrochloride was divided into two parts, one part of pyridoxine hydrochloride was considered as control (no Biofield Treatment was provided), while second part received The Trivedi Effect® - Biofield Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Alice Branton. The PXRD analysis exhibited that the relative intensities and crystallite sizes of the most characteristic diffraction peaks in the Alice’s treated pyridoxine hydrochloride were significantly decreased from 1.59% to 44.47% and 6.31% to 28.28%, respectively compared with the control sample. Overall, the average crystallite size of the treated sample was significantly reduced by 5.55% compared with the control sample. The particle size values at d10, d50, d90, and D(4, 3) (average mass-volume diameter) in Alice’s treated sample were significantly reduced by 13.87%, 9.95%, 7.87%, and 6.59%, respectively compared to the control sample. The specific surface area of treated sample was significantly increased by 13.67% compared to the control sample. The DSC analysis revealed that the melting point of the treated sample was lower by 0.20% with a significant reduction of the latent heat of fusion by 24.52% compared with the control sample. The TGA analysis revealed that the total weight loss in the treated sample was increased by 1.76% compared with the control sample. The DTG analysis indicated that the maximum thermal degradation temperature of the treated sample was reduced by 0.88% compared to the control sample. Overall, the thermal analysis indicated that the thermal stability of the treated pyridoxine hydrochloride was declined compared to the control sample. Thus, The Trivedi Effect® might lead to the production of a polymorphic form of pyridoxine hydrochloride which would be more soluble, absorbable, have a higher dissolution rate and be more bioavailable compared with the untreated sample. The Biofield Energy Treated pyridoxine hydrochloride would be beneficial in the design of better nutraceutical and pharmaceutical formulations which might provide better therapeutic responses against vitamin B6 deficiency, sideroblastic anemia, cardiovascular disease, diabetes, Alzheimer’s disease, pyridoxine-dependency seizures, metabolic disorders, pulmonary tuberculosis, hyperhomocysteinemia, cancer, anxiety, asthma, depression, dysmenorrhea, etc.