Learning Exercise

Assay Standard Curve

This module focuses on the generation of a standard curve based on the activity of an enzyme. A plasma cholesterol assay is used as an example.

Material:: Enzyme Kinetics
Date last modified:: September 29, 2009
Created by:: Scott Cooper
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Enzyme Kinetics

Six flash animations that show the data collection, graphing and calculations commonly done in an enzyme kinetic assay... see more

Material Type:: Tutorial
Author:: Carol Ann Paul, Wellesley College; Cecilia Yu, Wellesley College
User Rating:: 5 stars

Exercise

Two files are attached. The first is a powerpoint file that contains a self paced tutorial on how to generate a standard curve. The second file contains the excel spreadsheet used to generate the figures. This should not be given to the students, as it is what they are supposed to generate.

Additional Information: http://serc.carleton.edu/dev/introgeo/mathstatmodels/examples/34497.html

Disciplines

Audience

Topics

Assay Standard Curve

Type of Task

Learning Objectives

The goal of this module is to introduce the basic mathematics that describe enzyme kinetics. Students should become familiar with using spread sheets to calculate the amount of cholesterol in a sample.

Assessment

Pre and post-assessment questions are embedded in the powerpoint file.