Learning Exercise

Assay Standard Curve

This module focuses on the generation of a standard curve based on the activity of an enzyme. A plasma cholesterol assay is used as an example.

Six flash animations that show the data collection, graphing and calculations commonly done in an enzyme kinetic assay... see more


Two files are attached. The first is a powerpoint file that contains a self paced tutorial on how to generate a standard curve. The second file contains the excel spreadsheet used to generate the figures. This should not be given to the students, as it is what they are supposed to generate.


Assay Standard Curve

Learning Objectives

The goal of this module is to introduce the basic mathematics that describe enzyme kinetics. Students should become familiar with using spread sheets to calculate the amount of cholesterol in a sample.


Pre and post-assessment questions are embedded in the powerpoint file.